Cell-Free DNA Extraction Kit
Cell-Free DNA Extraction Kit
Ordering Information
| Catalog No. | Model | Format | Sample & Volume | Elution Volume |
|---|---|---|---|---|
| ZD-IL-M-10 | II-M | 10 preps/box | 1–5 mL serum/plasma | 5–20 µL |
| ZD-IL-M-20 | II-M | 20 preps/box | 1–5 mL serum/plasma | 5–20 µL |
| ZD-IL-M-40 | II-M | 40 preps/box | 1–5 mL serum/plasma | 5–20 µL |
| ZD-IL-MiniPlus-50 | II-MiniPlus | 50 preps/box | 1–2 mL serum/plasma | 20–50 µL |
| ZD-IL-MiniPlus-100 | II-MiniPlus | 100 preps/box | 1–2 mL serum/plasma | 20–50 µL |
| ZD-IL-M-5 | II-M | 5 preps/box | 40 mL urine | 5–20 µL |
| ZD-IL-M-10 | II-M | 10 preps/box | 40 mL urine | 5–20 µL |
| ZD-IL-M-15 | II-M | 15 preps/box | 40 mL urine | 5–20 µL |
Nucleic Acid Extraction or Purification Reagent (Zhejiang Medical Device Filing No. 20150181)
Product Introduction
Used for the extraction, enrichment, and purification of cell-free DNA from samples. The purified product is intended for clinical in vitro diagnostic use. Under the action of the buffer system, cell-free DNA is rapidly released from clinical samples, adsorbed onto the high-performance solid-phase matrix, and high-purity nucleic acid is obtained after elution.
Storage Conditions & Shelf Life
- Proteinase K should be stored at 2–8°C for long-term storage. The remaining components may be stored at ambient temperatures from −40°C to 40°C, with relative humidity not exceeding 75%, in a dark place free from corrosive gases.
- Shelf life: 24 months.
Required Equipment
Centrifuge, water bath. The YL-MiniPlus model kit additionally requires a vacuum aspiration device.
Product Features
- Uses a spin column format capable of processing 1–4 mL of plasma per extraction, ensuring sufficient DNA yield for downstream sequencing.
- Elution volume as low as 10 µL with high final concentration, greatly improving downstream workflow efficiency.
- High DNA extraction efficiency — recovery rate of fragments above 100 bp exceeds 80%.
- High-purity extracted DNA suitable for direct downstream applications such as sequencing library construction and qPCR.
Note: Clinical anticoagulated blood samples stored at 4°C for one week were used. The upper plasma layer was collected after mixing, centrifuged at 4000 RPM for 10 minutes, and the supernatant was taken. Cell-free DNA concentration was relatively high due to the extended storage time.
Lanes 1 and 2 show the recovery results after adding 15 µL DNA Marker to 2 mL plasma for extraction. Lanes 3 and 4 show 15 µL DNA Marker directly electrophoresed. Comparison of band intensities indicates recovery rate > 80%.
Comparison of extraction results against a competitor brand, analyzed on an Agilent 2100 Bioanalyzer.